Ausschwing-Rotoren

Ausschwingrotoren zeichnen sich durch eine lange Trennstrecke für gute Bandenseparation, minimale Wandeffekte und eine ausgezeichnete Bandenqualität aus.
Ausschwingrotoren ermöglichen es, dass Röhrchen den Zentrifugationswinkel während der Zentrifugation ändern. Die Zentrifugenbecher (buckets) sind im Stillstand vertikal und im Lauf horizontal angeordnet. Durch diese parallele Geometrie der Zentrifugenbecher wird es der Probe ermöglicht, sich ohne Unterbrechung durch die Dichtegradienten zu bewegen, im Gegensatz zur Festwinkelgeometrie, wo die Probe auf die Wand trifft, was die Trennung beeinträchtigen kann.

Ausschwingrotoren sind ideal zur Trennung großvolumiger Proben bei niedrigen Geschwindigkeiten und besonders hilfreich, wenn Proben in einem zonalen Dichtegradienten aufgelöst werden sollen.

Kennen Sie schon unseren neuen Intellifuge - Rotorkalkulator ? Wenn nicht, lernen Sie in diesem kurzen 2 Minuten Video  mehr über die Möglichkeiten zur Umrechnung und Konfigurierung ihrer Rotoren und  Röhrchen!


FAQ on Swinging-Bucket Rotors

Why use a swinging-bucket rotor for density gradient centrifugation?

Density gradient centrifugation requires samples to separate into distinct layers based on density. Because swinging-bucket rotors position tubes horizontally during operation, they help create uniform gradients and well-defined sample bands, making them a preferred choice for many gradient-based separation workflows.

What applications are swinging-bucket rotors commonly used for?

Swinging-bucket rotors are frequently used for density gradient centrifugation, blood processing, bioprocessing workflows, and when highly purified samples are required . They are often selected when maintaining sample integrity and achieving high separation quality are important.

How does a swinging-bucket rotor work?

In a swinging-bucket rotor, sample tubes begin in a vertical position and gradually move to a horizontal position during centrifugation. This orientation allows particles to travel evenly through the sample, making swinging-bucket rotors especially suitable for density gradient separations and applications that require distinct sample layers.

What is the difference between a swinging-bucket rotor and a fixed-angle rotor?

A swinging-bucket rotor holds samples horizontally during centrifugation, making it well suited for density gradient separations and applications that require well-defined sample layers. They can also be used for pelleting and result in the pellet forming at the very bottom of the tube. A fixed-angle rotor keeps tubes at a constant angle and is typically preferred for rapid pelleting applications. The best choice depends on the separation objective and sample type.

What is a swinging-bucket rotor?

A swinging-bucket rotor is a centrifuge rotor that allows sample buckets to pivot during centrifugation. As rotational speed increases, the buckets swing outward into a horizontal position, creating a consistent linear sedimentation path that supports efficient sample separation.